Resources/Equipment

 General Molecular Biology, Biochemistry, and Cell Biology Equipment

  • Centrifuges: Fisher Marathon Micro A, Eppendorf 5415C, Eppendrof 5402 refrigerated, Sorvall ST-8, Savant Speed Vac
  • General: Heatblocks, stir plates, balances, shakers, water bath, vortexers, etc.
  • DNA and protein electrophoresis and western blotting equipment 
  • Genesys UV/Vis spectrophotometer 
  • Applied BioSystems QuantStudio 5 Real-Time multi-color 384 well block real time PCR system
  • BioRad MyiQ single color real-time PCR detection system
  • BioRad GelDoc Imaging system
  • 4°C refrigerators, -20°C and -80°C freezers
  • Thermo Scientific MaxQ4450 incubated shaker
  • VWR Incu-Line and Fisher Isotemp bacteria incubators
  • Bead tissue lyser
Mammalian Cell Culture Incubators and Equipment
  • 2 Thermo Scientific Forma 370 Stericycle CO2 mammalian cell incubators
  • 2 Forma water-jacketed incubators
  • Eppendorf refrigerated centrifuge
  • Forma Tissue Culture Hood
Olympus Live-Cell Imaging System
  • Custom configured in collaboration with Olympus.  Housed in dedicated microscopy suite within the lab
  • Olympus IX-83 two-deck inverted microscope
  • 4x, 10x, 20x, 30x (silicone), 40x (LWD), 60x (oil) objectives
  • Differential interference contrast
  • Z-drift compensation (ZDC) laser focusing system (Olympus)
  • Lambda 10-2 dual filter wheels for ratiometric imaging with Smart Shutter (Sutter Instruments)
  • Filters for DAPI/SPQ, Fura-2, BCECF, FITC/GFP/Fluo-4/DAF-FM, TRITC/Cy3, CFP/YFP FRET/cameleon, SNARF (Chroma Technologies) 
  • Lambda LS 300 W Xe arc lamp (Sutter Instruments)
  • XCite LED 120 Boost LED light source
  • Olympus DSU spinning disk confocal with filters for DAPI, FITC, TRITC, Cy5 (Semrock), SNARF dual emission, and GFP/mCherry FRET   
  • Automated programmable stage (Prior)
  • Hamamatsu ORCA-Flash4.0 sCMOS camera (2Kx2K; 30 fps at full frame)
  • Tokai Hit (WSKM-F1) temperature-controlled and CO2-controlled incubation system for long-term time-lapse imaging of multi-well plates, including Neco control software and objective heater
  • Vibration isolation table (TMC) 
  • Heated (TC-344C), 6-channel perfusion system (Warner Instruments) for 35 mm glass bottom dishes (RC-37F) and coverslips (RC-26) with pinch valves
  • Peristaltic pump for perfusion
  • Bioptechs Delta-T heated chamber with perfusion lid and objective heater  
  • Imaging acquisition and analysis workstation (Dell Precision 7910 with Intel Xeon E5-2650 v3 processor, 32 GB RAM, 2x 512 GB SATA solid state drives, NVIDIA Quadro K2200 4GB GPU) with Metamorph Advanced for Olympus, MetaMorph NX 2.0, and MetaFluor for Olympus ratiometric imaging software, ImageJ, FIJI, Icy
  • Uses: Live-cell imaging of furs-2, fluo-4, DAF-FM, SNARF, BCECF, genetic GFP-based indicators, longer term imaging (5% CO2, 37 °C), 4-color immunofluorescence 
Nikon Imaging System ("Little Nikon")
  • Nikon TS100 inverted microscope
  • Phase contrast 10x and 100x objectives, 10x, 40x (LWD), 60x (LWD) PlanFluor objectives, 20x PlanApo objective
  • 50W HBO Hg Arc Lamp
  • XCite 110 LED light source with instant on/off for shuttering
  • GFP/DAF-FM/Fluo-4 filter set; Texas Red filter set
  • Photometric's Cool Snap HQ 12-bit cooled CCD camera
  • QImaging Retiga R1 USB3 cooled CCD camera
  • Color camera for histology
  • Micromanager (µManager) software for acquisition
  • Uses: Live cell imaging of GFP, calcium (fluo-4), nitric oxide (DAF-FM), green or red GECO, etc.
Nikon Imaging System ("Big Nikon")
  • Nikon Eclipse TE2000U inverted microscope
  • Prior Lumen Pro 2000S light source
  • Retina R6 CCD camera
  • Sutter excitation and emission filter wheels, smart shutter
  • Micromanager (µManager) software for acquisition
  • DAPI, CFP, GFP, YFP, TRITC, TexasRed, filters
  • Uses: Live cell imaging of GFP, calcium (fluo-4), nitric oxide (DAF-FM), green or red GECO, etc.; CFY/YFP FRET biosensors
TECAN Spark 10M Multi-Mode Plate Reader
  • Absorbance
  • Top and bottom fluorescence with monochrometer excitation and monochrometer/filter fusion optics
  • High resolution area scan
  • Enhanced PMT
  • Incubation heating with carbon dioxide and oxygen control (including hypoxia) for real-time, live cell measurements
  • Two channel injection system
  • NanoQuant Plate for DNA/RNA quantification
  • SparkControl Magellan software for kinetic analyses
  • Uses: Endpoint assays (ELISA); DNA-RNA quantification; Real-time, live cell imaging of proliferation (XTT), mitochondrial membrane potential (JC-1), H2O2 production (amplex red), calcium (fluo-4), etc.
BioRad Chemidoc MP gel imaging system
  • Cooled CCD, 6 megapixels 
  • Excitation options: Trans-UV (302 nm excitation), Epi-white, Trans-white, Trans-blue (450−490 nm ex), Epi-blue (460−490 nm ex), Epi-green (520−545 nm ex), Epi-red (625−650 nm ex), Epi-far red (650−675 nm excitation), Epi-IR (755−777 nm ex) 
  • Emission options: 590/110 nm filter for protein and DNA gel and chemiluminescence, 518–546 nm for blue-excitable fluorophores and stains, 577–613 nm filter for green-excitable fluorophores and stains, 675–725 nm for red-excitable fluorophores and stains, 700–730 nm filter for far red-excitable fluorophores and stains, 813–860 nm for near IR-excitable fluorophores and stains
Transepithelial Electrophysiology Equipment
  • World Precision Instruments DVC-1000 and Warner EC-825 dual channel epithelial voltage clamp amplifiers
  • Various Ussing chambers and accessories 
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