Protocols

Lab protocols:

• Hank's Balanced Salt Solution for live cell imaging
• Solutions for immunofluorescence of cultured cells
• Basic immunofluorescence protocol for cultured cells
• Loading of ALIs with DAF-FM for imaging of reactive nitrogen species
• Loading of primary ALIs with Fluo-4 for imaging calcium
• ALI transwell coating solution protocol
• Media for primary cells (Lonza-based)
• Stock solutions for nasal/bronchial gland work
• BacMam transduction (for cADDis) of 8-well chamber slides
• Lipofectamine 3000 for chamber slides
• SPQ Buffers for nitrate substitution
• Heat-killing of bacteria

Important Lab Info:

• Wide-field IX-83 microscope filters
• DSU microscope filters 
• Microscope configurations (fpbase.org)
• Genetically-encoded fluorescent biosensors in the lab